High Frequency Shoot Regeneration for Mass Multiplication of Desmodium gangeticum (L.) DC—An Important Anticancer, Antidiabetic and HepatoprotectiveEndangered Medicinal Plant

Preeti Srivastava; Brahma Deo Singh; Kavindra Nath Tiwari; Gaurava Srivastava

Volume : IV, Issue : VIII, August - 2015

High Frequency Shoot Regeneration for Mass Multiplication of Desmodium gangeticum (L.) DC—An Important Anticancer, Antidiabetic and HepatoprotectiveEndangered Medicinal Plant

Preeti Srivastava, Brahma Deo Singh, Kavindra Nath Tiwari, Gaurava Srivastava

Abstract :

Clonal multiplication protocol from in vitro culture of node and leaf explants from 4–week–old seedlings of Desmodium gangeticum (Fabaceae), a valuable, but endangered medicinal plant, was developed. An average of 24.1 shoots/ nodal explant were obtained after 4 weeks of culture initiationon MS medium containing 4.40 μM of BA; addition of 1.13μM PVP or ascorbic acid had a small promotory effect, while presence of NAA at 1.07, 2.68 or 10.72 μM tended to suppress shoot regeneration, except in the case of 8.90 μM BA plus 2.68 μM NAA, whichyielded 29.1 shoots/explant from 100% of explants. Multiple shoot (26 shoots/culture) regeneration was achieved from 84% of the cultures when callus derived from leaf explant was subcultured on medium supplemented with 28.90 μM BA and 2.68 μM NAA.The shoots were rooted by pulse treatment with 492.12 μM of indole–3–butyric acid for 30 min and then cultured on half–strength MS medium;~ 10 good quality roots/shoots were produced in 98% of shoots. Plantlets were successfully acclimatized and transferred to the field with survival rate of 92%, where they grew normally without any observable morphological variation. This protocol may help long–term conservation and mass propagation for commercial propagation and high biomass production of this valuable medicinal plant.