High Performance Liquid Chromatographic  method for simultaneous estimation of α–amyrin,  β–sitosterol and lupeol from dried whole plant  powder of Leptadenia reticulata (Retz.) Wight &  Arn. and Pluchea lanceolata (DC.) CB.

Dr. Vidya Dighe; Saikat S. Mallick; Hina Q. Shaikh

Volume : III, Issue : IV, April - 2014

High Performance Liquid Chromatographic method for simultaneous estimation of α–amyrin, β–sitosterol and lupeol from dried whole plant powder of Leptadenia reticulata (Retz.) Wight & Arn. and Pluchea lanceolata (DC.) CB.

Dr. Vidya Dighe, Saikat S. Mallick, Hina Q. Shaikh

Abstract :

A simple reversed phase high performance liquid chromatographic (RP-HPLC) method was established for simultaneous determination of alpha-amyrin, beta-sitosterol and lupeol in the two medicinally important plants, Leptadenia reticulata Wight & Arn. and Pluchea lanceolata (DC.) CB. Clarke. respectively. A good linear regression relationship between peak areas and concentrations was obtained over the range of 0.1�g/mL to 500�g/mL for three phytochemicals with correlation coefficient of more than 0.998 for all three phytochemicals. Precision, specificity and robustness studies were carried out, values less than two were observed. Recovery values from 98.15 to 98.94 indicated accuracy of the method was good.