HISTOCHEMICAL CHANGES IN THE GILLS OF THE FRESHWATER CRAB, BARYTELPHUSA CUNICULARIS (WESTWOOD): EXPOSED TO MALATHION

SHIPRA SINHA; GAJENDRA KUMAR YADAV; AKHILA SATISH; RUPALI TIWARI; SONAL NAGWANSHI

Volume : VIII, Issue : II, February - 2019

HISTOCHEMICAL CHANGES IN THE GILLS OF THE FRESHWATER CRAB, BARYTELPHUSA CUNICULARIS (WESTWOOD): EXPOSED TO MALATHION

Shipra Sinha, Gajendra Kumar Yadav, Akhila Satish, Rupali Tiwari, Sonal Nagwanshi

Abstract :

The terrestrial crab plays relevance modal in the study of population and also beneficial for human activities. As the crabs are abundantly available locally and used as diet for some people with great nutritive value, there is a potential to project it as poor man�s protein. The present study deals with the histopathological changes in the freshwater crab Barytelphusa cunicularis. Live specimens of Barytelphusa cunicularis were collected from local wet areas of Maroda sector–Bhilai, (C.G). Average weight of crabs varies from (45–50gms), the carapace length and carapace width varies from 3.20–4.50mm and 4.25–5.80mm respectively. The crabs were acclimatized in the laboratory condition, and kept in two groups the control group set free from Malathion and the experimental group was exposed to malathion for LC₅₀ at different concentration 0.45ppm, 0.30ppm, 0.26ppm and 0.25ppm for 24hrs, 48hrs, 72hrs and 96hrs respectively. The gills were dissected from both the control and experimental crabs and the sections of 5�m thick were cut, processed and stained with heamatoxylin and eosin. They were examined and studied under Olympus light microscopy by a built in camera. The histopathological changes suggest that there is thickening of gill lamella, rupture of capillaries, hyperplasia of epithelial cells were also seen, which leads to the fusion of gill lamella. This indicates that the Crabs play a significant modal in the aquatic environment and also good indicator for polluted condition.