Volume : VII, Issue : III, March - 2018

Histological effects of various fixatives on Small Intestine

Dr Ved Prakash, Dr. Muktyaz Hussein

Abstract :

 

The fixation is a critical step in the preparation of histological sections by which biological tissues are preserved from decay, thereby preventing autolysis or putrefaction.  This is of value in the microscopic examination of cells and tissue. The present study aimed to find the best fixative for a particular organ, so that the best histological section details can be produced. The five different fixatives namely 10% formaline, Bouin’s fluid, Carnoy’s fluid and Zenker’s fluid were used. The small intestine pieces were taken for study. We studied the effect of five different types of fixatives on small intestine namely 10% formalin, Buffered 10% formalin, Bouin’s fluid, Zenker’s fluid, Carnoy’s fluid and to observe the optimum result in a particular fixative in H&E sections. The best fixatives for architectural preservation are Carnoy’s fluid and Zenker’s fluid. Best Fixatives for small intestine are Carnoy’s fluid and Zenker’s fluid.

Keywords :

Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Dr Ved Prakash, Dr. Muktyaz Hussein, Histological effects of various fixatives on Small Intestine, INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH : Volume-7 | Issue-3 | March-2018

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