Homology Modeling and Structural  Analysis of DNA Binding Response  Regulator of Bacillus anthracis

Amajala K Chaitanya; Dr. I. Bhaskar Reddy; Ms. P. Minakshi; Mr. Kunal Zaveri; Dr. DSVGK. Kaladhar

Volume : II, Issue : VIII, August - 2013

Homology Modeling and Structural Analysis of DNA Binding Response Regulator of Bacillus anthracis

Amajala K Chaitanya, Dr. I. Bhaskar Reddy, Ms. P. Minakshi, Mr. Kunal Zaveri, Dr. Dsvgk. Kaladhar

Abstract :

DNA binding response regulator plays a vital role in the signal transduction and various cellular functions. It is widely expressed in various beings such as Mycobacterium tuberculosis, Streptococcus pneumoniae as well in Bacillus anthracis. As it is very essential protein involving in the two-component regulatory system metabolism, it can be considered as one of the drug target for the Anthrax disease. As the three dimensional structure is not resolved for B. anthracis DNA Binding response regulator, we made an attempt to model the protein structure by selecting appropriate template structure through Modeller software. The obtained homology modeled structure is subjected to secondary structure and transmemane prediction analysis. The modeled protein is further subjected to energy minimization & model evaluation studies with Swiss-Pdb Viewer & Procheck, Errat respectively. The energy minimized modeled protein is further subjected to structure superposition studies in which the target & template protein structures are aligned by computing the RMSD (Root Mean Square Deviation) value.