Immunohistochemical Evaluation of Estrogen and Progesterone Receptors and HER2 Expression Using Novel Rabbit Monoclonal Antibodies on Cell Blocks and Tissue Blocks  Sections of Breast Carcinoma

Dr Ram Nawal Rao; Divya Verma; Dr Narendra Krishnani; Dr Manoj Jain; Dr Amit Agrawal

Volume : VI, Issue : XII, December - 2017

Immunohistochemical Evaluation of Estrogen and Progesterone Receptors and HER2 Expression Using Novel Rabbit Monoclonal Antibodies on Cell Blocks and Tissue Blocks Sections of Breast Carcinoma

Dr Ram Nawal Rao, Divya Verma, Dr Narendra Krishnani, Dr Manoj Jain, Dr Amit Agrawal

Abstract :

Introduction: Fine needle aspiration (FNA) is a good modality of choice for early detection of east carcinomas. Assessment of estrogen and progesterone receptors (ER, PR) and HER2 expression by Novel Rabbit monoclonal antibodies SP1, SP2 and SP3 immunocytochemistry (ICC) have higher sensitivity than routinely used FDA–approved mouse monoclonal and polyclonal antibodies performed on cellblocks from FNA of east carcinoma as earlier reported in the studies. Materials and Methods: In this prospective study, we compared ER, PR and HER2 expression on cellblocks in east carcinomas using formalin–fixed FNA samples with formalin fixed resection tissue block sections. Results: 70 fresh FNA cell blocks from east carcinoma of primary and metastatic with subsequent resection tissue blocks were identified. All 70 cases were diagnosed as invasive ductal carcinoma on FNA. Few studies on east carcinomas using Novel Rabbit monoclonal antibodies have determined the validity of such testing using cellblocks with correlation of tissue bocks. This study revealed 100% correlation for positive and negative Her2/neu results between the cellblocks and tissue blocks sections. For ER testing the results showed 95.65% sensitivity, 100% specificity, 100% positive predictive value (PPV), and 96.42% negative predictive value (NPV). For PR testing the results showed 90.5% sensitivity, 100% specificity, 100% PPV, and 93.5% NPV respectively.

In conclusion:Immunocytochemistry for ER,PR and Her2/neu using rabbit monoclonal antibodies performed on formalin–fixed, paraffin–embedded cell blocks prepared from fresh FNA is most reliable methods in predicting the expression of these markers when correlated with immunohistochemistry (IHC) performed on the corresponding tumor tissue sections.