In Vitro Antibacterial Potential of Some Medicinal Plants Against Human Enteric Pathogens

Gajendiran K; Senthilkumar P K; Reetha D

Volume : III, Issue : VIII, August - 2014

In Vitro Antibacterial Potential of Some Medicinal Plants Against Human Enteric Pathogens

Gajendiran K, Senthilkumar P K, Reetha D

Abstract :

To investigate in vitro antibacterial potentials of five different medicinal plant leaf extracts against enteric pathogens. Five solvent were used Viz., hexane, chloroform, ethyl acetate, acetone and methanol extracts. Leaf extracts were evaluated against enteric bacterial pathogens by using standard disc diffusion, determination of minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC). Methanol extract of Vitex negundo leaves showed potent antibacterial activity (inhibition zone: 7.8-20.7 mm, MIC: 15.62 - 31.25 �g/mL, MBC: 31.25 -62.5 �g/mL) against all the pathogenic enteric bacteria (Escherichia coli, Salmonella typhi, Viio cholera, Viio fluvialis, Shigella flexneri, Proteus vulgaris and Proteus mirabilis) tested. For the first time it was observed that methanol extract of V. negundo leaves exhibited strong viiocidal activity in vitro conditions. Therefore, it will be useful to identify and isolate the active compounds of this extract that could be a good alternative medicine to treat cholera.