IN VITRO ASSESMENT OF TOLERANCE OF LACTOBACILLUS SPP. TO GASTROINTESTINAL TRACT ENVIORNMENT

Patil.S.B; Raorane C.J; Patil P.K; Sapale G.G.

Volume : IV, Issue : XI, November - 2015

IN VITRO ASSESMENT OF TOLERANCE OF LACTOBACILLUS SPP. TO GASTROINTESTINAL TRACT ENVIORNMENT

Patil. S. B, Raorane C. J, Patil P. K, Sapale G. G.

Abstract :

In order to act as a potential Probiotic, exogenously administered organism (belonging to Lactobacillus and Bifidobacterium species) have to pass through the gastrointestinal tract, and hence during their passage through gastrointestinal tract these organism must tolerate the high pH of the stomach and high bile salt concentration in the small intestine, also they must compete with the other microorganisms in the colon. In the present study five isolates of the Lactobacillus spp. isolated from the curd fermented idly batter and Cheese samples were studied for their tolerance to gastric pH, 0.3% bile salt, and also other characters like ability to utilize prebiotic and their antimicrobial action against the other intestinal inhabitant. All isolates were survived up to the 90 mins. In gastric pH (2.5) except isolate IV In addition these isolates survived and grew in the medium supply with 0.3% bile salt except isolate IV. Furthermore all isolates were found to reduce the cholesterol and exerted antimicrobial activity against common intestinal inhabitants. Isolates V was able to utilize Inulin as a prebiotic in addition to all above characteristics including Inulin utilization ability so it may be good candidate for Probiotic preparations.