In vitro conservation of some Drosera taxons from the protected areas of PNMA (Romania). Drosera anglica Hudson a specie critically endangered with extinction

Eliza Maria Agud

Volume : IV, Issue : XII, December - 2015

In vitro conservation of some Drosera taxons from the protected areas of PNMA (Romania). Drosera anglica Hudson a specie critically endangered with extinction

Eliza Maria Agud

Abstract :

Our research encompasses ample studies of in vitro conservation of some taxons from Drosera specie, endangered with extinction, from the protected area of PNMA (Romania). Drosera anglica Hudson specie is found in our country only in Transylvania in the Gil?u Mountains, a rarity of botany, a geoelement with poor populations, critically endangered (CR). Conservation through in vitro multiplication was made starting from the very young bud cultivated on the Murashige and Skoog, 1962 (MS) medium, with the variants: Vo = MS; V1= MS+0.5mg/l AIB+1.0mg/l BA; V2= MS+0.5mg/l AIB+1.0mg/l2iP; V3=MS+0.5mg/lAIB= 20mg/lAd.SO4 (table 1). After 45–50 days of in vitro culture we followed: the percentage of regeneration, rooting, multiplication and acclimatization (by evaluating the average of the number of neo–plantlets/explants). In vitro evolution is favoured by the presence of cytokinins in the medium, in the case of this experiment BA and 2iP, 1.0mg/lm and also of AdSO4 – 20mg/l (which can substitute cytokinins), combined with an auxine in a small dose (0,5mg/l AIB). After about 3 weeks of ex vitro preculture of the neo–plantlets there are obtained vigorous plants which can ensure the repopulation of the protected area through their planting in the area of origin.