Volume : IV, Issue : XII, December - 2015

In vitro propagation of Vanilla planifolia Andr. –a spice orchid

Saranjeet Kaur

Abstract :

This communication reports simple and effective in vitro protocol for in vitro propagation of Vanilla planifolia using uni–nodal stem segments in Mitra (M) medium and Murashige & Skoog (MS) medium alone and their combinations with cytokinins such as BA, Kn and auxin; NAA (1.0, 2.0 mgl–1) each. The regeneration response was obligatory to use of growth adjuncts in M medium.100% explants responded in both (M and MS) medium supplemented with BA (2 mgl–1) and a maximum of 6.0±0.81 and 6.0±0.86 shoots were obtained in M and MS medium respectively using BA (2 mgl–1). M medium with BA (2 mgl–1) favoured early plantlet development within 11.65±0.41 weeks. Extra axillary meristematic loci were induced opposite to the dormant axillary bud position in M+BA (1 mgl–1) supplemented medium. Higher concentration of BA (2 mgl–1) in the cultures allowed multiplication of shoot bud. NAA (1, 2 mgl–1) favoured callusing at basal part of shoot bud and initiating multiple shoot buds. The regenerated shoots rooted in their respective media combination developing into complete plantlets.

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Saranjeet Kaur In Vitro Propagation of Vanilla Planifolia Andr. -A Spice Orchid International Journal of Scientific Research, Vol : 4, Issue : 12 December 2015

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