In–vitro model showing Differentiation of Wharton’s Jelly derived Mesenchymal Stem Cells (MSCs) to Neuronal Phenotype through supplementation of Bacopa monneri: an in–vitro approach

Jyotsana Tyagi; Krupa Shah; Bhoomi Sastri; Mahesh Sharma; Rakesh Rawal

Volume : IV, Issue : VI, June - 2015

In–vitro model showing Differentiation of Wharton’s Jelly derived Mesenchymal Stem Cells (MSCs) to Neuronal Phenotype through supplementation of Bacopa monneri: an in–vitro approach

Jyotsana Tyagi, Krupa Shah, Bhoomi Sastri, Mahesh Sharma, Rakesh Rawal

Abstract :

Human umbilical cord Wharton�s jelly derived MSCs were induced to differentiate using an in–vitro approach. Study included exposure of MSCs to crude methanolic extract of Bacopa monneri and serum collected from healthy volunteers supplemented with Bacopa monneri as test samples. Cells were investigated for morphological changes and Nestin gene expression to prove its neuronal lineage against �–Mercaptoethanol (BME) as positive(+ve) control. Results indicated that Bacopa supplemented serum (BSS) showed greater differentiation capabilities as compared to +ve control. However, crude extract and un–supplemented serum (USS) didn�t reveal neither morphological differentiation nor Nestin expression. These results lead us to hypothesize that the fortified serum with secondary metabolites in BSS play a crucial role in MSCs differentiation towards neuronal phenotype.