In–vitro study to evaluate various drugs for potential effect on different cancer cell lines

Jaisen Madhav Lokhande; Sudhir Pawar

Volume : VII, Issue : I, January - 2018

In–vitro study to evaluate various drugs for potential effect on different cancer cell lines

Jaisen Madhav Lokhande, Sudhir Pawar

Abstract :

Abstract:

Background and Objectives: With the increasing cost of new drug development, the concept of drug repositioning can be considered as an alternate method. The present study evaluated the effects of various non anti–cancer drugs for their possible effects on cancer cell lines.

Methods: The selected drugs in 4 graded concentrations were evaluated against cancer cell lines using Sulforhodamine B assay. Parameters evaluated were concentrations required for 50% growth inhibition (GI₅₀), total growth inhibition (TGI) and lethal concentration in 50% (LC₅₀) of various cancer cell lines.

Results: Doxycycline showed GI₅₀against most of the cancer cell lines at concentrations which are achieved in human at standard doses. Other drugs which showed GI₅₀included metformin, gentamicin and azithromycin. None of the drugs achieved the LC₅₀.

Conclusion: Known drugs with suitable pharmacokinetics and pharmacodyanamics can be explored for other indications including cancers. Further studies are needed to validate these drugs in clinical settings.