Influence of Spirulina platensis Cyanobacterium on Some pathogenic bacteria isolated from different diets .

Abeer; A.Abu Zaid; Doaa; M. Hammad; Eman; M. Sharaf

Volume : IV, Issue : II, February - 2015

Influence of Spirulina platensis Cyanobacterium on Some pathogenic bacteria isolated from different diets .

Abeer, A. Abu Zaid, Doaa, M. Hammad, Eman, M. Sharaf

Abstract :

The present work was carried out to examine Spirulina Platenss water extract as antibacterial agent. Twenty bacterial strains (M01 -M20) were isolated under aerobic conditions from twelve traditional food products; further all strains were identified by partial 16SrRNA gene sequencing and phylogenetic analysis. Fifteen strains were identified by 97-100% identity including Bacillus circulance (4), Bacillus subtilis (4), Staphylococcus aureus (3), and Echerichia coli (4). Other isolates were identified by 85-92% identity; therefore these species are considered as new ones and named: Bacillus sp. (M07, M09, M10) and Staphylococcus sp. (M12, M13).Antibacterial activity of S. platensis water extract was recorded against the 20 bacterial strains. At S. platensis water extract concentration of 100mg/ml; inhibition zones diameter for all tested isolates ranged between 4 and 12 mm, while minimum inhibitory concentration of S. platensis extract(1�l/ml)was found to be effective against all Bacillus circulance, Esherichia coli strains and 2 out of 5 strains of Staphylococcus aureus. Results obtained revealed that water extracts of S. platensis showed better antibacterial activity against the pathogens used; further researches are recommended to identify and purify natural bioactive products from S. platensis as antimicrobial compounds, this will provide a new opportunity for the application of this cyanobacterium as a perfect probiotic, neutraceutical and in preventing some chronic diseases where free radicals are involved.