Volume : IV, Issue : VIII, August - 2015

Interference by Various Fractions of HbA1 while estimating total HbA1c% by High Performance Liquid Chromatography in Diabetics, if any?

Dr. Ketki Khandhadiya, Dr. Shailesh Kumar, Dr. Pragna Rao, Dr. Sohil Takodara, Mr. Manoj Kumar Yadav

Abstract :

Glycated Hb, measured as HbA1c is accepted as the gold standard marker for long–term glycemic control, for the preceding six to eight weeks. Aim of study was to measure total HbA1c% and fractions of HbA1 in diabetics and healthy controls, correlate total HbA1c% with fractions of HbA1 and fasting plasma glucose (FPG) levels in diabetics and healthy controls,to measure each fractions of HbA1 which might cause fluctuations in the value of HbA1c .The study was conducted in department of Biochemistry, Kasturba Medical College, Manipal.(n= 467).Total HbA1c and various fractions of HbA1 were estimated by high performance liquid chromatography method. Plasma glucose was estimated by hexokinase method.Total HbA1c%, HbF%,HbA1b% were significantly increased in diabetic patients compared to healthy controls . HbA1a% was not significantly increased in diabetic patients, compared to healthy controls. Weak positive correlation was found between total HbA1c% and FPG.HbF% was significantly increased in diabetic patients. Elevated HbF% in diabetic patients might be due to an intereference or cross reactivity of HbA1c% with HbF% during estimation, so HbF, which is actually a part of HbA1c is eluted out separately and which might lead to underestimation of HbA1c , it could result in sub¬optimal therapy and, thereby, increased risk of diabetic com–plications. FPG levels correlated less well with HbA1C%, which showed that HbA1c might be reliable on estimated average glucose (eAG) rather than FPG.

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Dr. Ketki Khandhadiya, Dr. Shailesh Kumar, Dr. Pragna Rao, Dr. Sohil Takodara, Mr. Manoj Kumar Yadav International Journal of Scientific Research, Vol : 4, Issue : 8 August 2015


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