Introgression of Broad spectrum blast resistance gene Pi2 into Mega variety Swarna through MABB

Rambabu. R.; Vijay kumar. S; Abhilash kumar. V; Aruna. J; Madhavi. K. R; Madhav. M.S; Ravindrababu. V; Srinivas Prasad. M

Volume : V, Issue : VIII, August - 2016

Introgression of Broad spectrum blast resistance gene Pi2 into Mega variety Swarna through MABB

Rambabu. R. , Vijay Kumar. S, Abhilash Kumar. V, Aruna. J, Madhavi. K. R, Madhav. M. S, Ravindrababu. V, Srinivas Prasad. M

Abstract :

Swarna is one of the most popular high yielding rice varieties in India. It had good grain quality and requires 25% less nitrogen as widely claimed by the farmers released in Andhrapradesh in 1982. It spread across the subcontinent and into Bangladesh, where it was never officially released. Despite, its superior grain and yield qualities swarna is highly susceptible to blast disease. Therefore, in the present study a blast resistant gene Pi2 from donor C101A51 were introgressed into Swarna through marker assisted backcross eeding (MABB) strategy. Twenty five pants were selected possessing Pi–2 gene in homozygous condition through foreground selection by gene specific marker AP5659–5 coupled with stringent phenotypic selection with virulent M.oryzae isolate (SPI–28). Four plants were selected through background selection analysis using polymorphic SSR markers with recurrent parent genome recovery (95%). The indentified plants were morphological similar to recurrent parent in relation to plant height and grain quality. However this introgressed lines yielded more than the recurrent parent.