Isolation and Identification of Amylase  Producing Bacterium from Domestic  Wastewater of Buldana District (M.s.)

Garode A. M.; Sonune N. A.

Volume : II, Issue : V, May - 2013

Isolation and Identification of Amylase Producing Bacterium from Domestic Wastewater of Buldana District (M.s.)

Garode A. M. , Sonune N. A.

Abstract :

Amylase is the most important industrial enzymes and having great significance in Biotechnological studies. Amylase production from bacteria is economical because the enzyme production rate is higher in bacteria as compared to other microorganism. The aim of this study was to isolate amylase producing bacteria from domestic wastewater. In this study cultural, morphological and biochemical characteristics of the bacterial isolates were studied. Total 40 bacterial cultures were isolated from collected domestic wastewater samples on nutrient agar medium. All isolates were screened for amylolytic activity by starch agar plate method. Among 40 bacterial isolates, only 8 isolates showed the best amylolytic activity. These isolates related to the genera Bacillus sp. and Paenibacillus sp.