Isolation and Identification of soil isolates  of Pseudomonas species via FAME analysis

Vijaya Dewaliya; Raashi Jasodani

Volume : II, Issue : IX, September - 2013

Isolation and Identification of soil isolates of Pseudomonas species via FAME analysis

Vijaya Dewaliya, Raashi Jasodani

Abstract :

Pseudomonas is a genus that contains over 40 species of bacteria; the genus is divided into five groups of classification. It is important genus of bacteria from clinical as well as environmental perspective. Most of the species of the genus harbour one or multiple plasmids. These plasmid impart various characteristics to the organisms like antibiotic resistance, catabolism of complex organic compound etc. The genus is also known for its lateral gene transfer and hence very important clinically. In present study soil samples were collected from the soil near to cotton industry effluent treatment, sugar mill and soil from automobile garage. Samples were screened for presence of Pseudomonas sp. on Cetrimide agar media. Gram staining, Biochemical tests and FAME analysis were carried out to confirm findings. The study gives insight into vast potential of Pseudomonas sp. in the field of biotechnology. It can be exploited for its diverse plasmid composition. These soil isolates posses ecological importance also, which can be further explored.