ISOLATION AND IDENTIFICATION OF WOUND PATHOGENS AND ANALYSIS ANTIBIOTIC SENSITIVITY USING ANTIBIOTICS AND SOAPS

PRIYA GOURI.G.; ASHA.R.; MUTHUSELVAM.M.; ANANDHI.K.

Volume : IV, Issue : VII, July - 2015

ISOLATION AND IDENTIFICATION OF WOUND PATHOGENS AND ANALYSIS ANTIBIOTIC SENSITIVITY USING ANTIBIOTICS AND SOAPS

Priya Gouri. G. , Asha. R. , Muthuselvam. M. , Anandhi. K. ,

Abstract :

In the present study 25 wound swabs were collected from burnt patients in Tiruchirappalli district Tamil Nadu. From the swabs bacterial species were isolated using Nutrient agar, MacConkey and Blood agar medium. The bacterial isolates were identified with the help of culture, morphological and biochemical characteristics. The bacterial isolates were confirmed as Streptococcus pyogenes, Proteus vulgaris, Pseudomonas aeruginosa and Staphylococcus aureus. The isolated bacterial species protein profile was also analyzed by SDS–PAGE. Seven bands were observed in Streptococcus pyogenes band range from 160–36 KDa. In bacteria, the maximum and minimum zone of inhibition was observed in antibiotic ceftriazone and clindamycin respectively. The highest antibacterial activity was noted De�scab, Deltol, and Lifebouy against all bacterial isolates. At the same time Lifebouy highly inhibit the growth of Proteus vulgaris (16�0.81 mm in diametery)