ISOLATION AND SCREENING OF LASPARAGINASE PRODUCING FUNGI FROM KADALUNDI MANGROVE FOREST SOIL

Vipina R; A. Vijaya Chitra

Volume : V, Issue : XI, November - 2016

ISOLATION AND SCREENING OF LASPARAGINASE PRODUCING FUNGI FROM KADALUNDI MANGROVE FOREST SOIL

Vipina R, A. Vijaya Chitra

Abstract :

L–asparaginase (E.C.3.5.1.1) has chemotherapeutic property and has been used in the treatment of acute lymphoblastic leukemia and lymphosarcoma. Microorganisms such as bacteria and fungi produce asparaginase and have wide application in medical and food industry. In the present study, eight fungal isolates were obtained from the soil samples collected from Kadalundi mangrove forest. The isolated fungi were further screened for production of L–asparaginase using Czapek�s Dox Agar medium using plate assay and among them 2 isolates showed significant enzyme activity. The isolates were identified as Aspergillus oryzae and Byssochalymus niveus and their L–asparaginase production was carried out under submerged fermentation. Aspergillus oryzae showed maximum enzyme production