Volume : IV, Issue : XII, December - 2015

ISOLATION, CHARACTERIZATION, OPTIMIZATION AND PURIFICATION OF PHB DEPOLYMERASE

Arpitaben Tulsibhai Patel

Abstract :

<p>The screening and isolation of PHB degrading microorganisms were done on the basis of the ability to produce extracellular PHB depolymerase on PHB containing BHM agar plates. Those isolates were selected which were produced clear zone on PHB agar plate due to hydrolysis of PHB. The optimal condition for PHB depolymerase production was found to be at 30 °C and pH 6 for fungal culture, initial substrate concentration of 0.2%(w/v). Higher % Weight loss of polymers was observed in case of shaking condition (12%) than static condition (7%). MP5 showed maximum PHB depolymerase production of 2.88 U/ml at 30°C and pH 6 with substrate concentration of 0.2%(w/v)</p>

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

ARPITABEN TULSIBHAI PATEL Isolation, Characterization, Optimization and Purification of Phb Depolymerase International Journal of Scientific Research, Vol : 4, Issue : 12 December 2015

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