ISOLATION, IDENTIFICATION, ENZYME PRODUCTIVITY, ANTIBACTERIAL ACTIVITY   AND   MOLECULAR   CHARACTERIZATION   OF INTESTINAL BACTERIA OF ORNAMENTAL FISH KOI CARP  (Cyprinus carpio var koi.)  AND IT’S ROLE ON GROWTH

A. Ananth.; M.R.Rajan; P.Sivakumar

Volume : IV, Issue : VI, June - 2015

ISOLATION, IDENTIFICATION, ENZYME PRODUCTIVITY, ANTIBACTERIAL ACTIVITY AND MOLECULAR CHARACTERIZATION OF INTESTINAL BACTERIA OF ORNAMENTAL FISH KOI CARP (Cyprinus carpio var koi.) AND IT’S ROLE ON GROWTH

A. Ananth. , M. R. Rajan, P. Sivakumar

Abstract :

The present study deals with the isolation, identification, enzymatic, antibacterial and molecular characterization of intestinal bacteria of Koi carp and its role on growth for a period of 45 days. Five distinct colonies were isolated from the intestine of Koi carp based on biochemical tests, enzymatic productivity and molecular characterization.The bacterium was identified as Bacillus sp and mass multiplied. Six different feeds having different concentration of Bacillus sp. such as feed I (Control), Feed II (1ml), Feed III (2ml), Feed IV (3ml), Feed V (4ml), and Feed VI (1ml yeast) were prepared by using fish meal, groundnut oilcake, wheat flour and tapioca flour and given to koi carp for a period of 45 days. The feed consumption, feed conversion ratio, feed conversion efficiency, growth, percentage growth, relative growth rate, assimilation, metabolism, gross growth efficiency and net growth efficiency was higher in feed V (4 ml of Bacillus sp.)