IJSR - International Journal of Scientific Research

Dr. Dinesh Kumar; Amit Kumar; Anupama Guleria; Aarti Kumari

Volume : III, Issue : II, February - 2014

Isolation, Immobilization and Characterization of Xylanase From A New Isolate Bacillus Atrophaeus E8

Dr. Dinesh Kumar, Amit Kumar, Anupama Guleria, Aarti Kumari

Abstract :

Xylanase is an industrially important enzyme which degrades xylan randomly and produces xylo-oligosaccharides, xylobiose and xylose. It has a role in bio-bleaching of paper pulp, increasing pulp ightness, additive in animal feedstock to improve its nutritional value and can be used for ethanol production. A new isolate E8 was isolated from degraded saw dust on potato dextrose agar plates at pH 5.2 at 30°C. This isolate was identified by using 16s rDNA amplification and sequencing and subsequent analysis using BLAST as Bacillus atrophaeus E8. The 16S rDNA gene sequence Bacillus atrophaeus E8 was deposited in GenBank with the accession number JX658789. Free xylanase showed optimum activity at pH 5.0 and 35°C temperature. Immobilized xylanase showed higher pH (6.0) and temperature (55°C) stability then free enzyme. Immobilized enzyme can be reused 2-3 times under assay conditions.