Isolation, Phylogenetic Identificationand  Proteolytic Activity of Bacillus Subtilis  Jskrsb1

Jeyasree Jayaraju; Dr. V. SUKUMARAN

Volume : II, Issue : VIII, August - 2013

Isolation, Phylogenetic Identificationand Proteolytic Activity of Bacillus Subtilis Jskrsb1

Jeyasree Jayaraju, Dr. V. Sukumaran

Abstract :

The present investigation to isolate and identification of Bacillus subtilis JSKRSB1 bacteria from soil sample were collected from mangrove forest region at Muthupettai Thiruvarur District, Tamil Nadu, India. .Partial sequencing of 16SrRNA gene is a good alternative for problematic phenotypic identifications and placing isolates in their right taxonomic position. The 16S rRNA gene had 961 nucleotide base pairs and it was closely related (99%) to the type strain of Bacillus (GenBank Accession Number KC631644). The use of isolates with enzymatic activities of potential advantage and biotechnological applications is considered important now that environmentalists are concerned with the safety which might be endangered by the release of genetically engineered microorganisms (GEMs) in the environment.