Isolation, Preliminary Screening and Process Optimization for Production of Surface Active Agent from Chlorella Pyrenoidosa by Non–Disruptive Method

Ms. Mira A. Londhe; Dr. Suhas D. Khambe; Dr. Sanjay P. Govindvar

Volume : III, Issue : VI, June - 2014

Isolation, Preliminary Screening and Process Optimization for Production of Surface Active Agent from Chlorella Pyrenoidosa by Non–Disruptive Method

Ms. Mira A. Londhe, Dr. Suhas D. Khambe, Dr. Sanjay P. Govindvar

Abstract :

Biosurfactants are structurally diverse group of surface active molecules, synthesized by microorganisms. In this research work algae are isolated from different natural aquatic habitats and identified for biosurfactant production. Genus Chlorella was one of the most promising identified algae. Pure strain of Chlorella pyrenoidosa NCIM No. 2738 was collected and ability of biosurfactant production was identified by performing different primary confirmation tests. Optimization of biosurfactant production was done which shown that carbon, nitrogen sources and PH affects on biosurfactant production. Partial extraction and purification was done by Silica gel Chromatography. Product was chemically analyzed by thin layer chromatography where presence of carbohydrates and lipids were confirmed by Rf value 0.75 and 0.7 for lipids after own spot development when exposed to iodine. Thus, primary structure of biosurfactant was confirmed in the form of glycolipids but it is necessary to characterize this product on different levels of identification.