IJSR - International Journal of Scientific Research

Dr. (Mrs.) M. R. Ingle

Volume : VII, Issue : IV, April - 2018

Lignocellulolytic enzyme production by soft -rot fungi isolated from decomposing coconut coir pith

Dr. Mrs. M. R. Ingle

Abstract :

Fungi were isolated from decomposing coconut coir and screened for their abilities to produce lignocellulolytic enzymes.The fungal strains that showed positive results were selected and utilized for production of extracellular lignocellulolytic enzymes viz., cellulase and laccase by submerged fermentation using defined media and culture conditions.The crude enzyme extracts were used for determining FPASe, endoglucanase, β–glucosidase and laccase enzyme activities. Trichoderma atrovirideexhibited highest FPAse (54.343 U/mL) and endoglucanase (58.973U/mL) enzyme activities whereas β–glucosidase activity was very low (32.323 U/mL).However, Aspergillus flavusshowed maximumβ–glucosidase activity (75.863U/mL). Of all the nine fungal isolates, only Trichodermaatroviride andT.erinaceumshowed laccase enzyme activity.