Volume : II, Issue : VI, June - 2013

Lipase from organic solvent tolerant Bacillus strain C5: Isolation and Identification

B. Rajesh, I. Bhaskar Reddy

Abstract :

Organic solvents are extremely toxic to microbial cells even at very low concentration of 0.1% (v/v). They disrupt the bacterial cell memane, affecting the functional and structural integrity of the cells The aim of the present work was to develop a bioprocess using specific and sensitive plate assay for true lipase producing bacterium capable of tolerating various organic solvents from spoiled coconut. The bacteria was identified as Bacillus species and it showed tolerance to both aromatic and aliphatic solvents with maximum tolerance for n-hexane. Bacteria showed maximum growth at 45°C, pH 8 and retained more than 80% of its initial activity upon exposure to various organic solvents.

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

B. Rajesh, I. Bhaskar Reddy Lipase from organic solvent tolerant Bacillus strain C5: Isolation and Identification International Journal of Scientific Research, Vol : 2, Issue : 6 June 2013

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