Low Density Lipoprotein (LDL) measurement by Direct method vs Formula method(Friedewald’s equation)

Niranjan Singh

Volume : V, Issue : XII, December - 2016

Low Density Lipoprotein (LDL) measurement by Direct method vs Formula method(Friedewald’s equation)

Niranjan Singh

Abstract :

Estimating LDL–C is an important aspect in assessing the Cardiovascular status of dyslipidemic subjects. Many methods are available for estimating LDL–C concentration. Most laboratories determine LDL–C by Friedewald�s formula. The aim of this study was to compare calculated LDL–C with direct homogenous assay, and to determine to what extent LDL–C level was under or overestimated when it was calculated using the formula compared with direct measurement of LDL–C. Our study included 156 patients with TG<400 mg/dL. LDL–C estimation was done by homogenous assay & also calculated using the Friedewald�s formula. The mean LDL–C levels were 102. � 37.7 and 94.4 � 36.7 for Direct LDL–C and Friedewald�s calculated LDL–C. A good correlation was found between the calculated LDL–C method and direct LDL–C method assay, i.e. F–LDL v/s D–LDL (r = 0.867). F–LDL correlated with D–LDL estimation at both TG level of <150mg/dL and TG level between 150–400mg/dL