Medium Optimization for The Production of Mycophenolic Acid by Penicillium Brevicompactum through Statistical Assessment At Shake Flask Level

Umesh Luthra; Sneha Khadpekar; Aditi Trivedi

Volume : III, Issue : VII, July - 2014

Medium Optimization for The Production of Mycophenolic Acid by Penicillium Brevicompactum through Statistical Assessment At Shake Flask Level

Umesh Luthra, Sneha Khadpekar, Aditi Trivedi

Abstract :

Penicillium evicompactum is an fungal isolate used for the production of mycophenolic acid (MPA). MPA is an immunosupressive agent also having antineoplastic, antifungal, antiinflammatory properties. One of the efforts carried out to meet the increasing demand of MPA and its cost effective production, is media optimization through statistical approach. To determine the best carbon and nitrogen source and also the type of casein enzyme hydrolysate to increase the MPA production, a categorical factorial experiment was conducted. Thereafter nine components were screened using Plackett Burman design which were then optimized using full factorial design and further by one variable at a time. Along with the conventional method like Analysis of Variance, Half Normal, Normal plot and Pareto chart were employed to screen the significant factors. Casein enzyme hydrolysate, and polypropylene glycol were found to be the most significant factors that increase the production by 50%. The optimum concentration of 25 g/l of casein enzyme hydrolysate and 1 g/l of PPG gave the maximum yield of 8.85 mg/gm.