Micro Propagation and Plant  Strengthening of Tissue Cultured Plants,  Inoculated with Several Bacterial Strains

Sunitha panigrahi; Dr. k.Aruna Lakshmi; Nida Mir

Volume : II, Issue : VIII, August - 2013

Micro Propagation and Plant Strengthening of Tissue Cultured Plants, Inoculated with Several Bacterial Strains

Sunitha Panigrahi, Dr. K. Aruna Lakshmi, Nida Mir

Abstract :

Rapid plant production has become a crucial factor in agriculture and horticulture. Most plants tend to lose their vitality due to various growth inhibitors. Micro propagation involves expeditious growth of large number of plants using tissue culture. During this process, In vitro propagation of plants is often associated with high mortality rate during ex vitro establishment phase. Plants when grown in vitro are provided with ideal conditions necessary for their growth, these plants do not have fully functional dermal coverings, they are very fragile, hence, are highly susceptible to growth depreciation when grown outside the lab. Thus hardening and acclimatization of in vitro grown plantlets is critical but necessary phase in micro propagation. When these plantlets are removed from the culture vessel, and exposed to the environment, they suffer high environmental stress, as the soil rhizosphere has several root inhabiting microbes and are known for their endophytic benefits, they reduce the environmental stress, but the In vitro raised plants were never exposed to the soil rhizosphere which in turn may turn be harmful to the root which would be suddenly invaded by the microorganisms. In this review you\'ll see that different bacteria have been used to acclimatize the tissue cultured plants with the soil microorganisms which in turn showed enhanced growth of the plants. Different soil samples were collected from various places, from which the six microorganisms were isolated, each one having its unique biological function. The plants were treated with Single parameter of six different bacteria�s v.i.z Rhizobium, VAM, Pseudomonas, PSB, Acetobacter and Azospirillum, in different proportions; they were inoculated onto the roots of the plantlets. The bacteria enter into a symbiotic relationship with the roots of these plants. Such bacterial induction helps the plant attain the necessary conditions for uninterrupted growth. The micro propagated plantlets were inoculated by these bacteria during the hardening process. The growth rate caused by each of the bacteria was observed and compared. Striking success in the terms of increment in root length, shoot length, leaf primordial and number of leaves was noted. It was seen that Rhizobium inoculated plantlets showed high frequency of growth and are proven to be the most profitable.