Volume : IV, Issue : V, May - 2015

Micrografting of fruit crops–A Review

Haseeb Ur Rehman, Manav Indra Singh Gill

Abstract :

 Micrografting was developed in 1980s and consists of the placement in aseptic conditions of a maintained scion  onto an in vitro grown rootstock. The results of in vitro micrografting and the plant material derived from it can  be further multiplied in tissue culture conditions or acclimatized to out door conditions. Micrografting is a technique that potentially can  combine the advantages of rapid in vitro multiplication with increased productivity that results from grafting, superior rootstock and scion  combinations. Among various methods of micrografting, slit or wedge grafting has been found most suitable in case of fruit crops. In vitro  shoot tips are better as compared to in vivo shoot tips for carrying out micrografting resulting in higher graft success, less contamination,  less vitrification, lower shoot tip necrosis and good vigour of micrografts. Media used for growing micrografted plantlets also affects grafting  success, vitrification, contamination and vigour. Use of appropriate medium depends on genotype and growing conditions. Modification of  basal medium formulation has been an effective means for achieving good graft success. The sucrose concentration of the nutrient medium of  grafted plants has been found to play a significant role. Besides these, scion length and rootstock development stage also influences graft success, vitrification, shoot tip necrosis, vigour and contamination. The use of pre–treated shoot apices not only markedly increases the grafting  success but also overcome problems encountered during the handling of in vitro micrografting that results in owning and drying of apices

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Haseeb Ur Rehman, Manav Indra Singh Gill Micrografting of fruit crops–A Review International Journal of Scientific Research, Vol : 4, Issue : 5 May 2015

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