Volume : VII, Issue : VIII, August - 2018

MODIFIED CARBAPENEM INACTIVATION METHOD FOR PHENOTYPIC DETECTION OF CARBAPENEMASE PRODUCTION AMONG ENTEROBACTERIACEAE

Dr Nilakshi Gupta, Dr. Bharati Dalal, Dr. K. K. Lahiri

Abstract :

Introduction:Carbapenemase–producing enterobacteriaceae (CPE) are increasing worldwide. Infections due to carbapenem–resistant organism have become a great concern for the clinicians due to the limited therapeutic options & so rapid and accurate detection of CPE is necessary for appropriate antimicrobial treatment and hospital infection control. The aim of this study was phenotypic detection of carbapenemase production  among  enterobacteriaceae by modified carbapeneme inactivation method recommended by CLSI 2017.

Material and Methods: In this test colonies were transferred to tryptic soya oth meropenem (10μg) disk was added & incubated for 4 hours. Muller Hinton Agar plate was lawn cultured with E. coli ATCC 25922 as for Disc Diffusion test. Meropenem disk was removed & placed on E. coli lawn & incubated overnight.

Results:

1) Out of 180 isolates 51 (28.33%) were carbepenemase producing.

2)Incidence of carbepenemase producing organism was more in isolates from IPD(85%) as compared to isolates from OPD(15%) patients.

3)Incidence of carbapenem resistance organism is more in isolates from ICU (41.1%).

4) Among carbapenem resistant isolates,  15.51% was carbapenem resistant E.coli and  56.5% was carbapenem resistant Klebsiella.

Discussion & Conclusion: The mCIM is a simple, inexpensive, accurate, and

reproducible method for the identification of carbapenemase production among

Enterobacteriaceae where automated system are not available.

Keywords :

Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Dr Nilakshi Gupta, Dr. Bharati Dalal, Dr. K.K. Lahiri, MODIFIED CARBAPENEM INACTIVATION METHOD FOR PHENOTYPIC DETECTION OF CARBAPENEMASE PRODUCTION AMONG ENTEROBACTERIACEAE, INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH : Volume-7 | Issue-8 | August-2018


Number of Downloads : 358


References :