Molecular characterization of TLR2 gene exons and its association with SCS in HF crossbred cattle.

Jadhav; P.V.; Das; D.N.; Ramesha; K.P.

Volume : V, Issue : V, May - 2016

Molecular characterization of TLR2 gene exons and its association with SCS in HF crossbred cattle.

Jadhav, P. V. , Das, D. N. , Ramesha, K. P.

Abstract :

Present study was carried out to investigate genetic polymorphism of TLR2 gene exon 1 and exon 2 in 214 HF crossed cow. Molecular characterization of TLR2 gene was carried out by PCR– SSCP analysis. The study revealed presence of six SNPs viz., A827G, C1088G, C2155A, G2281C, G2410A and C2600T in exon 2 of TLR2 gene. SNP G2410A and C2600T were found to be significantly associated with SCS (p< 0.1+). It was found that for SNP G2410A, genotype GG (LSM for SCS 3.089 � 0.197) showed lower SCS than the genotype AA (LSM for SCS 3.375 � 0.176) and AG (LSM for SCS 3.447 � 0.197). Similarly for SNP C2600T, genotype CC (LSM 3.089 � 0.197) showed moderately significantly lower SCS (p< 0.10+) than the genotype TT (LSM 3.375 � 0.176) and CT (LSM for SCS 3.447 � 0.197). In conclusion, it is suggested that TLR2 gene could be a candidate gene associate with resistance to mastitis for molecular marker–assisted selection and eeding