Volume : IX, Issue : IV, April - 2020

Molecular diagnosis of human papilloma virus and its high risk type 16 by end point PCR

Dr. Deepak Kumar

Abstract :

Background and Aim: High risk Human papilloma virus (HPV) type16 is a causative agent in 60 % of cervical cancer and 93% of oropharyngeal cancer. Therefore, present study intended for the rapid diagnosis of HPV DNA and its type 16. Material and Methods: The confirm HPV type16 DNA positive samples were obtained for optimizing the PCR test. The PCR for the detection of β–globin DNA (Internal control), HPV DNA, HPV type16 was performed. The sensitivity and specificity of the assay were also tested. Results: The specific strong band for β–globin, HPV DNA and type 16 of size 268, 450 and 217 bp respectively has been observed on agarose gel while band was absent in negative control. The presence of band on diluting (1:100) the DNA sample and no band in HBV DNA (non–HPV DNA) sample confirm the sensitivity and specificity of the assay. Conclusion: Molecular assay for detection of high risk type16 of HPV using end point one step PCR is highly sensitive, specific rapid and cost effective which can be routinely used for screening of this high risk HPV type.

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Article: Download PDF    DOI : 10.36106/ijsr  

Cite This Article:

MOLECULAR DIAGNOSIS OF HUMAN PAPILLOMA VIRUS AND ITS HIGH RISK TYPE 16 BY END POINT PCR, Dr. Deepak Kumar INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH : Volume-9 | Issue-4 | April-2020

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