Molecular Identification Candida isolates from HIV positive patients in India

Ritu Goyal; Ravinder Kaur; Megh S. Dhakad; Absarul haque; Gauranga Mukhopadhyay; Preena Bhalla; Richa Diwan

Volume : IV, Issue : X, October - 2015

Molecular Identification Candida isolates from HIV positive patients in India

Ritu Goyal, Ravinder Kaur, Megh S. Dhakad, Absarul Haque, Gauranga Mukhopadhyay, Preena Bhalla, Richa Diwan

Abstract :

The objective of the study was to identify, characterize the Candida isolated from HIV/AIDS patients. Relevant clinical samples were collected from 200 HIV/AIDS patients. 143 yeasts were isolated and subjected to speciation by the conventional and molecular methods. We performed the Southern blot hyidization with radioactive labelled CARE–2 probes and random amplification of polymorphic DNA (RAPD). Patients ranged from17–68 years with a mean age of 33.83�9.07 years. Conventional methods identified C.albicans(82.51%), C.tropicalis(4.89%), C.krusei(4.19%), C.parapsilosis(1.49%), C.glaata(3%), C.zeylanoids(0.69%), C.kefyr(2.1%), C.lusitaniae(0.69%), Rhodotorula(1.4%). Further C.albicans was reconfirmed by DNA fingerprinting method and Candida non–albicans by RAPD method. DNA fingerprint patterns showed that isolates collected from different patients over a period of time were different compared to some which were related; they were mostly identical only in different body locations of the same patient. DNA fingerprint patterns indicate that the source of infection is not common to all patients and suggesting that commensal or endogenous isolates might be turning into pathogens. Early accurate diagnosis of the source of Candidiasis is needed for better understanding and clinical management in HIV/AIDS patients.