Volume : VII, Issue : IV, April - 2018

Neuronal delivery of small interfering (siRNA) by cationic liposome complex (CLC) in vitro and in vivo.

Dharamveer Singh, Jyotiumrao, Tanzeem Khan, Suman Yadav, Rambha Tripathi, Swati Saxena, Manjaribaluni, Snehaghildiyal, Atul Garg, Aloknath, A. K. Shukla, Tapan N Dhole

Abstract :

 Objective: Small interfering RNA (siRNA), effectively used for therapy against several diseases. Transfection of macromolecule in neuronal cells is

very hard. We used a N2A cells as neuronal model to optimize the siRNA transfection. The study optimized the synthesis of cationic liposome used for
the transfection of siRNA followed by determination of silencing efciency.
Method: In the study we used Neuro 2A (N2A) as neuronal model and Hep2 as non–neuronal model. RVG and RV–MAT peptide were commercially
synthesized and chemically tagged with FITC. N2A and Hep2 were cultured in DMEM respectively. BALB/c mice have been used as in vivo model.
Results: Liposome were prepared by cationic lipid (DOTMA) and neutral lipid (DOPE), 1:1 molar ratio was found optimal for maximum positive
charge on particle (r = 0.9977, P< 0.001, 95% CI). Particles achieved particle size below 100 nm in diameter (r = –0.9925, p = 0.0075, 95% CI).
Cationic liposomal complex (CLC) specically deliver siRNA in N2A cells and 100 pmole of GAPDH specic siRNA signicantly silence the
expression of mRNA. CLC having GAPDH specic siRNA specically reduced the expression in ain tissue only as compared to other organ in vivo.

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Dharamveer Singh, JyotiUmrao, Tanzeem Khan, Suman Yadav, Rambha Tripathi, Swati saxena, ManjariBaluni, SnehaGhildiyal, Atul Garg, AlokNath, A. K. Shukla, Tapan N Dhole, Neuronal delivery of small interfering (siRNA) by cationic liposome complex (CLC) in vitro and in vivo., INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH : Volume-7 | Issue-4 | April-2018


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