Nucleotide Sequencing and Analysis of –Subunit (accD region) of Acetyl–CoA carboxylase gene isolated from Nannochlropsis salina, Dunaliella tertiolecta and Tetraselmis suecica

Dr. Prakasam Velu; Dr. Elumalai Sanniyasi

Volume : IV, Issue : III, March - 2015

Nucleotide Sequencing and Analysis of –Subunit (accD region) of Acetyl–CoA carboxylase gene isolated from Nannochlropsis salina, Dunaliella tertiolecta and Tetraselmis suecica

Dr. Prakasam Velu, Dr. Elumalai Sanniyasi

Abstract :

We have designed primers for β – Subunit (accD region) of Acetyl–CoA carboxylase gene isolated from indigenous strains of Nannochlropsis salina, Dunaliella tertiolecta and Tetraselmis suecica. The primers were designed based on closest microalgal sequences that exist in database. Three subunits of ACCase were encoded by a nuclear genome and accDregion is encoded by plastid genome. It is the functional and conserved region of acc (Acetyl–CoA Carboxylase) gene. In our studies we observed, Nannochloropsis salina had 866 bp of accD region which is smaller than Dunaliella tertiolecta and Tetraselmis suecica. Hence the transcriptional rate of accD gene in N. salina much faster than other microalgae; it directly influences the fatty acid anabolic pathway. The pairwise alignment showed N. salina and T. suecica has 60% than other pairs.