Optimized Protocols for Extraction of  DNA in Plant and Blood Tissues

Ednaldo da Silva Filho; Taianara Tocantins Gomes Almeida

Volume : II, Issue : XI, November - 2013

Optimized Protocols for Extraction of DNA in Plant and Blood Tissues

Ednaldo Da Silva Filho, Taianara Tocantins Gomes Almeida

Abstract :

There is a variety of works in the molecular biology of plants and animals using different methods of DNA extraction. Some procedures are performed using commercial kits or protocols using different buffered solutions and denaturing agents (protein and polysaccharide), which makes the method very laborious and polluting to environment with a high economic cost. This scientific note has objective to demonstrate two optimized simple methods, less polluting to the environment with little formation of waste, less expense in detected by UV spectrophotometry through of 230/260 and 260/280 rations demonstrating materials and a lot of quality. Samples of different kinds of vegetables and blood (human and bovine) were tested for protocols 3% CTAB and precipitation with salt methods, respectively. Results were that pure DNA is possible to be acquired with few contaminations