Volume : VI, Issue : VI, June - 2017

Partial Purification of Galactosidase Extracted from Apricots (Prunus armeniaca kaisa)

Dr. Preeti Lahiri

Abstract :

 β Galactosidases are enzymes which has an increasing biotechnological and medicinal application for lactose hydrolysis and oligosaccharides synthesis. The β–galactosidase produced from apricot seeds was extracted and purified by precipitation with ammonium  sulphate  (30–70%) and dialysis was carried out at 40 C  for 24hr. The crude extract had a higher specific activity (6.19 U/mg proteins) which indicates a high enzyme yield. Purification of crude extract which was done with ammonium  sulphate (30–70%) increased the specific activity to 18.49 U/mg proteins  which was improved by dialysis to 21.61 U/mg proteins. The enzyme presented an optimum activity at pH 8.5 and 45°C.  The enzyme activity obtained was almost stable between pH 5.0 and 6.5.    

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Dr.Preeti Lahiri, Partial Purification of Galactosidase Extracted from Apricots (Prunus armeniaca kaisa), INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH : VOLUME-6 | ISSUE-6 | JUNE-2017

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