Partial purification of Glutathione–S–transferases in Plutella xylostella (L.)  show imparting resistance against  Flubendiamide

Dipali B. Borkar; V. L. Bagde; S. S. Munje; M.P. Moharil

Volume : II, Issue : IX, September - 2013

Partial purification of Glutathione–S–transferases in Plutella xylostella (L.) show imparting resistance against Flubendiamide

Dipali B. Borkar, V. L. Bagde, S. S. Munje, M. P. Moharil

Abstract :

Present investigation was undertaken to study Partial purification of Glutathione–S–transferases in imparting resistance in Plutella xylostella (L.) against Flubendiamide. To know the molecular mechanism of flubendiamide resistance in DBM, It was selected against flubendiamide. The LC50 of flubendiamide was 0.38 ppm at F5 generation which increased to 6.35 ppm in F10 selected generation resulting 16.62 fold resistance increased as compared to F5 generation. In partial purification by ammonium salt precipitation susceptible strain showed GST activity 2.94 fold pure and In resistance strain 6.96 fold pure by ammonium salt precipitation. 60–100 percent ammonium sulphate precipitated fraction 1 showed high specific activity as compared to fraction 2. Resistance strain showed 52 fold purity and susceptible strain showed 9 fold purity. From this concluded that fraction 1 showed high moleculer weight containing high specific activity.