Phenotypic and genotypic study of macrolide, lincosamide and streptogramin B (MLSB) resistance in clinical isolates of Staphylococcus aureus

Umamageswari S.S.M; Anantharaman C; Shameem Banu A.S; Kalyani M.

Volume : V, Issue : VIII, August - 2016

Phenotypic and genotypic study of macrolide, lincosamide and streptogramin B (MLSB) resistance in clinical isolates of Staphylococcus aureus

Umamageswari S. S. M, Anantharaman C, Shameem Banu A. S, Kalyani M.

Abstract :

Emergence of Methicillin resistant Staphylococcus aureus (MRSA) emphasizes the need for alternative thera? peutic options. So macrolide, lincosamide and streptogramin (MLSB) antibiotics are used to treat Staphylococcus aureus with preference to Clindamycin. In vitro routine tests for clindamycin susceptibility may fail to detect inducible clindamycin resistance due to erm genes resulting in treatment failure, thus necessitating the need to detect such resistance by a simple D test on a routine basis. The purpose of this study was to determine the frequency of cMLSB, iMLSB, and MS phenotypes using D?Test and PCR. Our study showed MRSA–33.1% and iMLSB – 14.11%. 9 strains of iMLSB had ermA gene detected by PCR. This study showed that D test should be used as a mandatory method in routine disc diffusion testing to detect inducible clindamycin resistance in Staphylococci for the optimum treatment of patients.