Phenotypic and Molecular Characterization of Extended-Spectrum ?-Lactamase producing clinical isolates of Klebsiella pneumoniae

Kiran Beri; Jayanthi G; Malini Shariff

Volume : VII, Issue : VI, June - 2018

Phenotypic and Molecular Characterization of Extended-Spectrum ?-Lactamase producing clinical isolates of Klebsiella pneumoniae

Kiran Beri, Jayanthi G, Malini Shariff

Abstract :

Introduction:K. pneumoniae, often associated with nosocomial infections, has acquired resistance against β–lactam antibiotics. Production of β–lactamase is considered as one of the most common mechanism that accounts for the resistance. It is known to produce significant percentage of ESBLs. Thus, the present study was aimed to characterize ESBL producing isolates of K. pneumoniae.

Material & methods: Sixty strains of K.pneumoniae were collected from patients visiting VPCI. Isolates were subjected to antibiotic susceptibility, phenotypic and genotypic confirmationof ESBL genes.

Results: Isolates exhibited (31–100%) resistance to various antibiotics. 53.33% tested positive using phenotypic confirmatory test. 91.7% isolates were positive for various ESBL genes.The most prevalent ESBL genotypes were bla_TEM–1, bla_SHV–1 and bla_CTX–M–1.

Conclusion: Multiplex PCR is sensitive and rapid method for identifying the ESBL genes. The prevalence of MDR ESBL producing K. pneumoniae appears to be high and majority of them tested positive for TEM–1, SHV–1 and CTX–M–1.