Phytochemical Screening and Tlc Profiling of Seeds of Crotalaria Verrucosa Linn

P. Kamalakar; G. Prabhakar; K. Shailaja

Volume : III, Issue : IX, September - 2014

Phytochemical Screening and Tlc Profiling of Seeds of Crotalaria Verrucosa Linn

P. Kamalakar, G. Prabhakar, K. Shailaja

Abstract :

Seeds of Crotalaria verrucosa L. were screened for their phytochemical constitution following hot continuous and successive extraction by Soxhlet apparatus. Qualitative assay and thin layer chromatography was done using a range of solvents. Extraction process was carried using different solvents successively in the order of increasing polarity. Qualitative analysis of the extracts using standard procedures, revealed the presence of alkaloids, flavonoids, tannins, saponins, glycosides, triterpenoids, phenols, steroids, coumarins, cardiac glycosides and phytosterols. TLC profiling of the extracts was performed using different solvent compositions, which yielded a wide array of compounds. Distinct qualitative manifestation of phytochemicals in screening test and chromatography of the seeds has demonstrated their potential for future drugs.