Prevalence and Antimicrobial Susceptibility of  Pyogenic Microorganisms Isolated from Clinical  Specimens of Patients of Lower Respiratory  Infection

Dr Mitesh H Patel; Dr Avani M Patel; Dr Nidhi Sood; Dr Dhara Modi; Dr Manisha Jain; Dr Gordhan Nakum

Volume : II, Issue : X, October - 2013

Prevalence and Antimicrobial Susceptibility of Pyogenic Microorganisms Isolated from Clinical Specimens of Patients of Lower Respiratory Infection

Dr Mitesh H Patel, Dr Avani M Patel, Dr Nidhi Sood, Dr Dhara Modi, Dr Manisha Jain, Dr Gordhan Nakum

Abstract :

Background :New emerging pathogens and associated antimicrobial resistance mechanisms have been observed in the respiratory tract of patients suffering from pyogenic infection in the last decade. Prevalence rate of metallo–β–lactamase (MBL)–producing Pseudomonas aeruginosa strains, Carbamapenase producing enterobacteriaceae, is growing, apart from prevalent ESBL positive E.coli & Klebsiella pneumoniae. Methods :In this study, 250 respiratory samples (Sputum) of were collected during a 24 months period. Microbiological cultures and antimicrobial susceptibility tests of the most frequently isolated bacteria were performed by standard disk diffusion method as per CLSI guidelines. Results : Out of 250 sputum samples, 142 pathogenic bacteria were isolated and characterized. Among the bacteria, Streptococcus pneumonia, Staphylococcus aureus (both MSSA & MRSA) & P. aeruginosa (Both MBL producing & Non MBL producing) showed highest prevalence. Conclusions : The detection of MBL–producing P. aeruginosa and MRSA in patients confirms that antimicrobial resistance patterns should be always kept under surveillance. Moreover hygiene regulations in pulmonary clinics should prevent a further spread of resistant bacterial strains.