PREVALENCE OF ESBL PRODUCING OXIDASE POSITIVE NON FERMENTING GRAM NEGATIVE BACTERIA ISOLATED FROM VARIOUS CLINICAL SPECIMENS AT A TERTIARY CARE HOSPITAL IN CHENNAI

Karthiga L; Srivani Ramesh

Volume : V, Issue : XI, November - 2016

PREVALENCE OF ESBL PRODUCING OXIDASE POSITIVE NON FERMENTING GRAM NEGATIVE BACTERIA ISOLATED FROM VARIOUS CLINICAL SPECIMENS AT A TERTIARY CARE HOSPITAL IN CHENNAI

Karthiga L, Srivani Ramesh

Abstract :

Oxidase positive nonfermenting bacteria (NFGNB) have emerged as potential nosocomial pathogens and are becoming resistant to commonly used antimicrobial agents, therefore the present study was under taken to investigate the prevalence of Extended Spectrum Beta Lactamases (ESBLs) production in various species of oxidase positive Non Fermenting Gram Negative Bacteria (NFGNB). Isolates of oxidase positive NFGNB from various clinical specimens were obtained from Department of Microbiology, Madras Medical College & Hospital, Chennai and speciated using conventional biochemical methods and antibiotic susceptibility testing was done as per CLSI guidelines. ESBL production was detected by phenotypic confirmatory disc diffusion test (PCT). ESBL phenotypes were subjected bla TEM screening using PCR. Out of 40 isolates from various clinical samples, Pseudomonas aeruginosa was the commonest accounting for 70% followed by Pseudomonas fluorescens 15%. Among the 40 isolates, 35% were multidrug resistant, 27.5% of the isolates were found to be ESBL producers. ESBL producers were negative for bla TEM gene by PCR assay. To conclude significant percentage of NFGNB were found to be ESBL producers in our study. To know the actual incidence of ESBLs in these organisms, all the NFGNB should be speciated and screened for ESBL production to avoid the spread of drug resistance.