Prevalence of Gram negative bacilli showing resistance to carbapenems

Dr Nibandhana; K. Usha; E. Kumar; Dr Usha Kalawat

Volume : V, Issue : XI, November - 2016

Prevalence of Gram negative bacilli showing resistance to carbapenems

Dr Nibandhana, K. Usha, E. Kumar, Dr Usha Kalawat

Abstract :

Carbapenems are the β–lactam antibiotics used in the treatment of infections due to multidrug–resistant gram negative bacteria. The aim of study is to determine the prevalence of carbapenemase producing gram negative bacteria phenotypically among various clinical isolates received in the microbiology laboratory in a tertiary care hospital. Double disk diffusion test and Modified Hodge test (MHT) were employed for carbapenemase detection. Out of 849 isolates, 145 were resistant to meropenem and 92 were resistant to imipenem by disc diffusion method. 117 positive by MER–EDTA, 75 positive for IMP–EDTA and 79 positive for MHT. Use of both MHT and combined disk test with EDTA as a screening method can increase sensitivity of detection of carbapenemases and can aid timely intervention to initiate infection control practices and thereby improving the outcome of patient