Prevalence of Neisseria Gonorrhoea Infection by Pcr Method Using Urine Samples From Hiv Sero–Positive Women

Okonkwo; E.C.; Ogbu; O.; Anyim; C.; Nworie; O.; Orji; J.O.; Nwuzo; A.C.

Volume : III, Issue : VIII, August - 2014

Prevalence of Neisseria Gonorrhoea Infection by Pcr Method Using Urine Samples From Hiv Sero–Positive Women

Okonkwo, E. C. , Ogbu, O. , Anyim, C. , , Nworie, O. , Orji, J. O. , Nwuzo, A. C.

Abstract :

Neisseria gonorrhoeae infection was diagnosed in human immunodeficiency virus (HIV) sero-positive women in Ebonyi state, Nigeria. The samples were screened for HIV at the laboratory of Applied Microbiology, Ebonyi State using diagnostic dipstick, while PCR study was carried out at university of Ibadan. Species specific primers were designed for the specific amplication of a 260bp region of the N. gonorrhoeae porA pseudo gene based on the numbering from strain MSII. All strains of N.gonorrhoeae tested were successfully detected by the designed PCR primer giving a single predicted product of 260bp amplicon in gel electrophoresis. The primer could not amplify DNA extracted from other Neisseria species. HIV status was established using rapid immunochromatographic assays as per national standards. Out of the total number of 105 urine samples, N. gonorrhoeae was detected in 16 (15.2%). There was significant association between N. gonorrhoeae infection and age but infection was not related to occupation and marital status. Analysis showed that N. gonorrhoeae infection is significantly associated with HIV status (15.2% vs 100%). N. gonorrhoeae is known to facilitate HIV transmission, hence there is the need for routine testing and treatment of all positive cases of infection to control HIV