PRODUCTION OF LIPOLYTIC ENZYMES BY ENDOPHYTIC FUNGI AND ITS USE IN THE HYDROLYSIS OF CRUDE OIL.

Jos� Moraes; Raimundo Barbosa–Junior; Luciana Xavier; Antonio Carvalho; Maria Sarquis; Artur Silvaand; Alberdan Santos

Volume : IV, Issue : VI, June - 2015

PRODUCTION OF LIPOLYTIC ENZYMES BY ENDOPHYTIC FUNGI AND ITS USE IN THE HYDROLYSIS OF CRUDE OIL.

Jos Moraes, Raimundo Barbosa Ndash Junior, Luciana Xavier, Antonio Carvalho, Maria Sarquis, Artur Silvaand, Alberdan Santos

Abstract :

Endophytic fungi were isolated from the seeds of oil palm (ElaeisguineensisJacq) by biomonitoring lipase activity on semi–solid media with chromogenic substrate. Molecular and classical methods were used to identify the species, and the selected fungus was confirmed as Penicilliumcitrinum. Method for thermal stability of the enzyme carried on crude oil hydrolysis. The fermentation was carried out in shake flasks and results were compared with control at 30�C and pH 6.8 Induction of lipase activity promoted a concentrated fraction of extra–cell lipase (FOL) with 137mg/L and activity of 12000 U/L in submerged culture. The hydrolysis yielded YP/ So = 0.4623 of free fatty acids. It was not observed that crude oil inhibit lipase activity, only products as known, whereas refined oil enhanced lipase activity. The micro–organism showed a production of a robust enzyme with prospects for technological application.