IJSR - International Journal of Scientific Research

Ch.V.Satya; D. Sri Rami Reddy

Volume : VI, Issue : VIII, August - 2017

PRODUCTION, PURIFICATION AND CHARACTERIZATION OF LASPARAGINASE BY ISOLATED ASPERGILLUS NIGER B4 UNDER SOLID STATE FERMENTATION USING SOYA AS SUBSTRATE

Ch. V. Satya, D. Sri Rami Reddy

Abstract :

The objective of this investigation was to isolate soil and marine fungal isolates and to screen them for L–asparaginase production. Three isolates out of 48 fungal strains were isolated among which Aspergillus niger B4 was selected for optimization of fermentation parameters employing Soya powder as substrate under solid state fermentation.The fermentation studies indicated that the maximum L–asparaginase production 227.63 U/gds was achieved at 96h fermentation period with pH 6.5, inoculum volume 15%v/w, moisture content 100%, incubation temperature 30^oC and particle size 0.6cm. The purified L–asparaginase was used for the characterization.