Protease Inhibition Studies and Metallic  Responses of Cucurbita maxima and  Citrullus lanatus Seed Coat Extracts

Mr. Sreenu Barla; Dr. DSVGK Kaladhar; Dr. Govinda Rao Duddukuric

Volume : II, Issue : IX, September - 2013

Protease Inhibition Studies and Metallic Responses of Cucurbita maxima and Citrullus lanatus Seed Coat Extracts

Mr. Sreenu Barla, Dr. Dsvgk Kaladhar, Dr. Govinda Rao Duddukuric

Abstract :

The impact of medical specialty activities with medicative properties to elucidate mechanisms will scale back unwellness risk. This experimentation has been conducted to seek out the proteolytic enzyme restrictive impact of Cucurbita maxima and genus Citrullus lanatus seed coat extracts. The restrictive action of enzyme and Protease–K has not shown any restrictive impact. Chymotrypsin inhibition has been determined at 10�l of crude extract. Pumpkin seed coat crude extract has shown enzyme (at 10�l) and Chymotrypsin (10 to 0.625�l) inhibition. All the tested concentrations has not shown any Protease–k inhibition. The isolated Cucurbita maxima and genus Citrullus lanatus seed coat extracts has not shown any inhibition with Trypsin and Protease–k however shown inhibition with Chymotypsin at 30%, 50% and 90% concentrations. FeSO4, Na2EDTA and (CH3COO)2Pb has shown Trypsin inhibition. All the metals except FeSO4, Na2EDTA and (CH3COO)2Pb has not shown any Trypsin inhibition. Typsin inhibition has not been seen once genus Citrullus lanatus seed coat extract (10�l) has been else before adding metal. Thus this shows the competitive binding of Citrullus lanatus seed coat extract. All the metals has not shown any Chymotrypsin inhibition. Addition of genus Citrullus lanatus seed coat extract has shown competitive inhibition in Phosphate buffer, MgSO4, HgCl2 and Na2EDTA. Protease–k has shown inhibition with FeSO4 and not shown any inhibition with different metal compounds. Competitive inhibition of genus Citrullus lanatus seed coat extract has been found in CuSO4 and HgCl2. Competitive binding of the FeSO4 with protease–k has been determined. Typsin inhibition has been seen once Cucurbita maxima seed coat extract (10�l) has been else before adding HgCl2. Thus this shows the binding of extract. All the metals haven�t shown any trypsin inhibition. Addition of Cucurbita maxima seed coat extract has shown competitive inhibition with all the metals in chymotrypsin. Protease–k has not shown inhibition with all metal compounds of Cucurbita maxima seed coat.