Protective Effects Of Phyllanthus Emblica In Cyclophosphamide Induced Genetic Damage In In Vitro Human Lymphocytes

Rudrama Devi .K; Keshava Rao K; Minny Jael. P

Volume : V, Issue : XII, December - 2016

Protective Effects Of Phyllanthus Emblica In Cyclophosphamide Induced Genetic Damage In In Vitro Human Lymphocytes

Rudrama Devi . K, Keshava Rao K, Minny Jael. P

Abstract :

Cyclophosphamide (CP) is a commonly used chemotherapeutic and immunosuppressive agent which is used in the treatment of wide range of cancers and autoimmune diseases. Besides that it is a well known carcinogen. In this study by using chromosomal aberrations (CA) s method, the modulatory effects exerted by the extract of phyllanthus emblica against the CP induced genotoxicity in the human lymphocyte various doses of PFE was used i.e 33, 78,114 ug/ml in human lymphocyte cultures. PFE has not induced any cytogenetic damage in human lymphocyte cell cultures. Hence it is found to be non mutagenic. However when cell cultures were treated with 100 �g/ml of CP induced the significant increase in the percentage of chromosomal aberrations but when primed with PFE to cell cultures there was a significant decrease in the frequency of chromosomal aberrations in invitro human lymphocytes. ?us the data clearly indicate that amla is a safer plant extract to be used along with CP in chemotherapy regimen